This intensive course of one week is organised by the Erasmus Optical Imaging Centre. It gives an overview of the rapidly developing advanced fluorescence imaging methods and how they are used in biomedical research. An important part is the practical work each afternoon in which you get the opportunity for real hands-on experience. The focus of the practicals is on functional imaging (time lapse, FRAP, FRET) and super resolution (SIM, STED and single molecule tracking).